Experiments – G MARS Pharma Solution

SECTION A

Introduction & Basic Concepts

Experiment #1

Introduction to Pharmacology, Experimental Pharmacology, its Scope, Objectives, and Foundational Concepts

ACTIVE

Experiment #2

Study of Commonly Used Instruments in Experimental Pharmacology

ACTIVE

Experiment #3

Study of common laboratory animals in experimental pharmacology.

ACTIVE

Experiment #4

Introduction to in-vitro pharmacology and physiological salt solutions

ACTIVE

Experiment #5

Study of dose calculation and stock solution preparation in pharmacological experiments.

ACTIVE

Experiment #6

Preclinical Experiments: Types and Details (In Vivo, In Vitro, Ex Vivo)

ACTIVE

SECTION B

Ethics, Handling & Techniques

Experiment #7

Maintenance of laboratory animals as per CPCSEA (CCSEA) guidelines (handling & restraining).

ACTIVE

Experiment #8

Common laboratory techniques: Blood withdrawal; Serum and plasma separation; Anaesthetics and euthanasia for animal studies.

ACTIVE

Experiment #9

Study of different routes of drug administration in small animals (rats/mice).

ACTIVE

SECTION C

Autonomic & Peripheral Pharmacology

Experiment #10

Effect of drugs on rabbit eye (pupil size / intraocular pressure).

ACTIVE

Experiment #11

Effect of drugs on frog’s ciliary motility in frog oesophagus.

ACTIVE

Experiment #12

Effect of agonists and antagonists on guinea pig ileum.

ACTIVE

Experiment #13

Effect of saline purgative on frog intestine.

ACTIVE

Experiment #14

Effect of spasmogens and spasmolytics using rabbit jejunum.

ACTIVE

Experiment #15

Effect of Drugs on Gastrointestinal Motility in Mice/Rats

ACTIVE

SECTION D

Central Nervous System (CNS)

Experiment #16

Effect of skeletal muscle relaxants using Rota-rod apparatus.

ACTIVE

Experiment #17

Effect of drugs on locomotor activity using actophotometer.

ACTIVE

Experiment #18

Study of stereotype behavior of drugs on rat/mice.

ACTIVE

Experiment #19

Study of Anti-Catatonic (Anti-Cataleptic) Activity in Rats/Mice

ACTIVE

Experiment #20

Anticonvulsant Activity by Maximal Electroshock Seizure (MES) or PTZ Method

ACTIVE

Experiment #21

Study of anxiolytic activity of drugs using elevated plus maze method.

ACTIVE

Experiment #22

Taming and hypnosis potentiating effect of chlorpromazine in mice/rat.

ACTIVE

SECTION E

Cardiovascular & Renal Pharmacology

Experiment #23

Study of isolation and perfusion of frog heart.

ACTIVE

Experiment #24

Study of effect of calcium ions and potassium ions on isolated frog heart

A. Study of effect of potassium ions on isolated frog heart

ACTIVE

B. Study of effect of calcium ions on isolated frog heart

ACTIVE

Experiment #25

Study of effect of acetylcholine and adrenaline on isolated frog heart

A. Study of effect of acetylcholine on isolated frog heart

ACTIVE

B. Study of effect of adrenaline on isolated frog heart

ACTIVE

Experiment #26

Effect of drugs on blood pressure and heart rate of dog.

ACTIVE

Experiment #27

Study of diuretic activity of drugs using rats/mice.

ACTIVE

SECTION F

Analgesic & Anti-Inflammatory

Experiment #28

Evaluation of analgesic activity by hot plate method.

ACTIVE

Experiment #29

Evaluation of analgesic activity by tail flick method.

ACTIVE

Experiment #30

Evaluation of anti-inflammatory activity using carrageenan-induced paw edema model.

ACTIVE

Experiment #31

Test for pyrogens (rabbit method).

ACTIVE

SECTION G

Endocrine & Metabolic Pharmacology

Experiment #32

Study of insulin-induced hypoglycaemic effect in rabbit.

ACTIVE

SECTION H

Gastrointestinal & Anti-Ulcer

Experiment #33

Study of anti-ulcer activity using pylorus-ligated (Shay) rat model and NSAIDs-induced ulcer model.

ACTIVE

SECTION I

Anaesthetics & Allergy Models

Experiment #34

Study of different types of Local Anasthetic Techniques

A. Study surface anaesthetic activity by loss of corneal reflex in rabbit

ACTIVE

B. Study infiltration anaesthetic activity by loss of cutaneous sensation in guinea pig wheal

ACTIVE

C. Study conduction anaesthetic activity by loss of reflex withdrawal in frog

ACTIVE

Experiment #35

Evaluation of anti-allergic activity by mast cell stabilization assay.

ACTIVE

Experiment #36

Effect of diphenhydramine in experimentally produced asthma in Rabbit

ACTIVE

SECTION J

In-Vitro Bioassay Studies

Experiment #37

Introduction to bioassay & assembly setup.

ACTIVE

Experiment #38

Dose-response curve (DRC) of acetylcholine using frog rectus abdominis muscle.

ACTIVE

Experiment #39

Effect of physostigmine and atropine on DRC of acetylcholine using frog rectus abdominis muscle & rat ileum respectively.

A. Effect of physostigmine on DRC of acetylcholine using frog rectus abdominis muscle & rat ileum.

ACTIVE

B. Effect of atropine on DRC of acetylcholine using frog rectus abdominis muscle & rat ileum.

ACTIVE

Experiment #40

Bioassay of histamine using guinea pig ileum by matching method.

ACTIVE

Experiment #41

Bioassay of oxytocin using rat uterine horn by interpolation method.

ACTIVE

Experiment #42

Bioassay of serotonin (5-HT) using rat fundus strip by three-point method.

ACTIVE

Experiment #43

Bioassay of acetylcholine using rat ileum/colon by four-point method.

ACTIVE

Experiment #44

Determination of PA₂ value of prazosin using rat anococcygenus muscle (Schild’s plot method).

ACTIVE

Experiment #45

Determination of PD₂ value using guinea pig ileum.

ACTIVE

SECTION K

Toxicology Studies

Experiment #46

Determination of acute oral toxicity (LD₅₀) of a drug from given data.

ACTIVE

Experiment #47

Determination of acute skin irritation/corrosion of a test substance.

ACTIVE

Experiment #48

Determination of acute eye irritation/corrosion of a test substance.

ACTIVE

SECTION L

Biomechanics & Biostatistics

Experiment #49

Estimation of serum biochemical parameters using semi-autoanalyser.

ACTIVE

Experiment #50

Calculation of pharmacokinetic parameters from given data.

ACTIVE

Experiment #51

Biostatistical methods in experimental pharmacology: Student’s t-test; ANOVA; Chi-square test; Wilcoxon signed-rank test.

ACTIVE

Explore Our Virtual Pharmacology Experiments

Introduction & Basic Concepts

Introduction to Pharmacology, Experimental Pharmacology, its Scope, Objectives, and Foundational Concepts

Study of Commonly Used Instruments in Experimental Pharmacology

Study of common laboratory animals in experimental pharmacology.

Introduction to in-vitro pharmacology and physiological salt solutions

Study of dose calculation and stock solution preparation in pharmacological experiments.

Preclinical Experiments: Types and Details (In Vivo, In Vitro, Ex Vivo)

Ethics, Handling & Techniques

Maintenance of laboratory animals as per CPCSEA (CCSEA) guidelines (handling & restraining).

Common laboratory techniques: Blood withdrawal; Serum and plasma separation; Anaesthetics and euthanasia for animal studies.

Study of different routes of drug administration in small animals (rats/mice).

Autonomic & Peripheral Pharmacology

Effect of drugs on rabbit eye (pupil size / intraocular pressure).

Effect of drugs on frog’s ciliary motility in frog oesophagus.

Effect of agonists and antagonists on guinea pig ileum.

Effect of saline purgative on frog intestine.

Effect of spasmogens and spasmolytics using rabbit jejunum.

Effect of Drugs on Gastrointestinal Motility in Mice/Rats

Central Nervous System (CNS)

Effect of skeletal muscle relaxants using Rota-rod apparatus.

Effect of drugs on locomotor activity using actophotometer.

Study of stereotype behavior of drugs on rat/mice.

Study of Anti-Catatonic (Anti-Cataleptic) Activity in Rats/Mice

Anticonvulsant Activity by Maximal Electroshock Seizure (MES) or PTZ Method

Study of anxiolytic activity of drugs using elevated plus maze method.

Taming and hypnosis potentiating effect of chlorpromazine in mice/rat.

Cardiovascular & Renal Pharmacology

Study of isolation and perfusion of frog heart.

Study of effect of calcium ions and potassium ions on isolated frog heart

Study of effect of acetylcholine and adrenaline on isolated frog heart

Effect of drugs on blood pressure and heart rate of dog.

Study of diuretic activity of drugs using rats/mice.

Analgesic & Anti-Inflammatory

Evaluation of analgesic activity by hot plate method.

Evaluation of analgesic activity by tail flick method.

Evaluation of anti-inflammatory activity using carrageenan-induced paw edema model.

Test for pyrogens (rabbit method).

Endocrine & Metabolic Pharmacology

Study of insulin-induced hypoglycaemic effect in rabbit.

Gastrointestinal & Anti-Ulcer

Study of anti-ulcer activity using pylorus-ligated (Shay) rat model and NSAIDs-induced ulcer model.

Anaesthetics & Allergy Models

Study of different types of Local Anasthetic Techniques

Evaluation of anti-allergic activity by mast cell stabilization assay.

Effect of diphenhydramine in experimentally produced asthma in Rabbit

In-Vitro Bioassay Studies

Introduction to bioassay & assembly setup.

Dose-response curve (DRC) of acetylcholine using frog rectus abdominis muscle.

Effect of physostigmine and atropine on DRC of acetylcholine using frog rectus abdominis muscle & rat ileum respectively.

Bioassay of histamine using guinea pig ileum by matching method.

Bioassay of oxytocin using rat uterine horn by interpolation method.

Bioassay of serotonin (5-HT) using rat fundus strip by three-point method.

Bioassay of acetylcholine using rat ileum/colon by four-point method.

Determination of PA₂ value of prazosin using rat anococcygenus muscle (Schild’s plot method).

Determination of PD₂ value using guinea pig ileum.

Toxicology Studies

Determination of acute oral toxicity (LD₅₀) of a drug from given data.

Determination of acute skin irritation/corrosion of a test substance.

Determination of acute eye irritation/corrosion of a test substance.

Biomechanics & Biostatistics

Estimation of serum biochemical parameters using semi-autoanalyser.

Calculation of pharmacokinetic parameters from given data.

Biostatistical methods in experimental pharmacology: Student’s t-test; ANOVA; Chi-square test; Wilcoxon signed-rank test.

Explore Our
Virtual Pharmacology
Experiments